Basic personal information

Name: Xiaolong-Shao, PHD

Position: Associate Professor

Organization: Nanjing Agricultural University

Office address: B634, Science building, No.1 Weigang, Nanjing, Jiangsu, 210095, China

Telephone:19968559752

E-mail: xlshao@njau.edu.cn

Academic qualifications/Academic visits

09/2016 – 06/2019, Ph.D. in Microbiology, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin China.

09/2013 – 06/2016, M.S. in Microbiology, College of Life Sciences, Northwest University, Xi’an, Shaanxi, China.

09/2009 – 06/2013, B.S. in Biological Science, College of Bioengineering and Biotechnology, Tianshui Normal University, Tianshui, Gansu, China.

Work experience

10/2021.10–now, Assistant Professor, College of Plant Protection, Nanjing Agricultural University, China

06/2019-09/2021, Postdoctoral Fellow, Department of Biomedical Sciences, City University of Hong Kong, HKSAR, China.

Project experience:

1.  National Natural Science Foundation of China Grants (32272619), 2023.01-2026.12

2.  National Natural Science Foundation of China Grants (32172358), 2022.01-2025.12

3.  National Natural Science Foundation of China Grants (31870116), 2019.01-2022.12

4.  National Natural Science Foundation of China Grants (31670127), 2017.01- 2020.12

5.  Nanjing Agricultural University high-level talent introduction research project (804140), 2021.12-2026.11

6.  Jiangsu Province Superior Discipline Project (80900246), 2021.12-2022.12

7.  Shenzhen Science and Technology Innovation Fund Project (JCYJ201708118103038049), 2018.04-2020.04

Primary research field

1. Disaster mechanism of plant bacterial diseases and green prevention and control technology

2. Co-evolution and adaptability mechanism of "biocontrol bacteria, pathogenic bacteria and plants"

3. Creation and application of new pathogenic bactericidal agents and biocontrol agents

Participated in the compilation of books

1. Wang B., Shao X.,  Qian G. (2023). Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope. In:  Minamino, T., Miyata, M., Namba, K. (eds) Bacterial and Archaeal Motility. Methods in Molecular Biology,  vol 2646. Humana, New York, NY.

2. Shao Xiaolong, Qian Guoliang. (2023). Identification and functional mechanism of novel biocontrol bacteria, Technical Guide for Molecular Microbiology Experiments (In press).

Invention patents

1. Qian Guoliang; Shao Xiaolong; Wang Limin; A biocontrol pantobacter KBA33 for inhibiting Kiwifruit ulcer and its application, 2023-10-20, China, 2023113710563

2. Qian Guoliang; Lin Long; Shao Xiaolong; Wang Limin; A microbial composition of OH11W and ZK-1 and its application, 2023-06-08, China, 202310677627X

3. Qian Guoliang; Lin Long; Wang Limin; Shao Xiaolong; A strain of Chrysobacterium biocontrol with "contact bactericidal" activity, 2023-10-20, China, 2023113685504

Social Service Work:

Reviewer for Molecular Plant Pathology 

Reviewer for Phytopathology Research

Reviewer for Genes and Diseases

Reviewer for Molecular Diversity

Reviewer for BMC Infectious Diseases

Reviewer for Archives of Microbiology

Reviewer for Blue Biotechnology

Reviewer for Infectious Disorders - Drug Targets

New Plant Protection (NPP) Youth Editorial Board

Academic editor of Chinese Science and Technology Knowledge

Guest editor of Journal of Visualized Experiments

Guest editor, Plants, on Special Issue: Occurrence and Control of Plant Bacterial Diseases

Agronomy Guest Editor, Special Issue: Genetic Diversity and Pathogenic Mechanisms in Plant Pathogenic Bacteria

List of representative papers (#co-first authors，*corresponding author)

1. Yao C, Ding Y, Liu J, Zhang Y, Han L, Liu N, Chen F, Qian G, Shao X*, and Deng X*. (2024) Global Functional Characterizations of Hypothetical Proteins Reveal Novel Virulence Factors in Pathogenic Bacterium. Nature Communications. (Under revision).

2.  Shao X, Wu Q, Li L, He W, He X, Cheng D, Murero A, Lin L, Wang L*, Zhong C, Huang L, Qian G. (2023) Adapting the inoculation methods of kiwifruit canker disease to identify efficient biocontrol bacteria from branch microbiome. Molecular Plant Pathology, 00, 1-12. (IF: 4.9)

3.  Lin L, Yang Z, Tao M, Shen D, Cui C, Wang P, Wang L, Jing M, Qian G*, Shao X*. (2023) Lysobacter enzymogenes prevents Phytophthora infection by inhibiting pathogen growth and eliciting plant immune responses. Frontiers in Plant Science. 14:1116147. (IF: 6.6)

4. Xiong D, He X, He W, Shen D, Wang L, Lin L, Minamino T, Shao X*, Qian G*. (2023) Loss of flagella-related genes enables a non-flagellated, fungal-predating bacterium to strengthen the synthesis of an antifungal weapon. Microbiology Spectrum. 2023 Jan 11:e0414922. (IF: 3.7)

5. Shao X*, Yao C, Ding Y, Qian G, He M, Deng X*. (2022) The transcriptional regulators of virulence for Pseudomonas aeruginosa: Therapeutic opportunity and preventive potential of its clinical infections. Genes and Diseases. Available online 1 October 2022 (IF: 6.8)

6.  Shao X#, Tan M#, Xie Y, Wang T, Huang H, Zhang Y, Liu J, Hua C, Zhang W, Wang X*, Deng X*. (2021) Integrated Regulatory Network in Pseudomonas syringae Reveals Dynamics of Virulence. Cell Reports. 2021 Mar 30;34(13):108920. (IF: 9.4)

7. Yao C#, Shao X#, Li J and Deng X*. (2021) Optimized Protocols for ChIP-seq and deletion mutant construction in Pseudomonas syringae. STAR protocols. 2021.100776.

8.  Shao X#, Zhang W#, Umar M#, Xie Y, Wang T, Zhang Y, Yang L, Kwok C*, Deng X*. (2020) RNA G-Quadruplex Structures Mediate Gene Regulation in Bacteria. mBio. Jan 2020, 11 (1) e02926-19. (IF: 7.9）

9.  Shao X, Xie Y, Zhang Y, Liu J, Ding Y, Wu M, Wang X, Deng X*. (2020) Novel therapeutic strategies for treating Pseudomonas aeruginosa infection. Expert Opinion on Drug Discovery. 15(12):1403-1423. (IF: 6.1)

10. Huang H#, Shao X#, Xie Y#, Wang T, Zhang Y, Wang X*, Deng X*. (2019) An integrated genomic regulatory network of virulence-related transcriptional factors in Pseudomonas aeruginosa. Nature Communications. 10(1):2931. (IF: 12.1)

11. Shao X, Xie Y, Zhang Y, Deng X*. (2019) Biofilm Formation Assay in Pseudomonas syringae. Bio-protocol. 9 (10): e3237.

12. Shao X#, Zhang X#, Zhang Y, Zhu M, Yang P, Yuan J, Xie Y, Zhou T, Wang W, Chen S, Liang H*, Deng X*. (2018) RpoN-dependent direct regulation of quorum sensing and the type VI secretion system in Pseudomonas aeruginosa PAO1. Journal of Bacteriology. 200 (16), 00205-18. (IF: 3.2）

13. Wang J#, Shao X#, Zhang Y, Zhu Y, Yang P, Yuan J, Wang T, Yin C, Wang W, Chen S, Liang H*, Deng X*. (2018) HrpS is a global regulator on T3SS and non-T3SS genes in Pseudomonas savastanoi pv. phaseolicola. Molecular Plant-Microbe Interactions. 31 (12), 1232-1243. (IF: 4.2）

14. Lin L, SHAO X, Yang Y, Murero A, Wang L, Xu G, Zhao Y, Han S, Su Z, Xu K, Yang M, Liao J, Li K, Liu F*, Qian G*. (2024) Lysobacter enzymogenes: a fully armed biocontrol warrior. Journal of Integrative Agriculture. https://doi.org/10.1016/j.jia.2024.02.021.

15. Liao J, Li Z, Xiong D, Shen D, Wang L, Lin L, Shao X, Liao L, Li P, Zhang LQ, Wang HH, Qian G*. (2023) Quorum quenching by a type IVA secretion system effector. ISME Journal. 2023 Jun 20. (IF: 11.0)

16. Wang B, Zhang Z, Xu F, Yang Z, Li Z, Shen D, Wang L, Wu H, Li T, Yan Q, Wei Q, Shao X, Qian G*. (2023) Soil bacterium manipulates antifungal weapons by sensing intracellular type IVA secretion system effectors of a competitor. ISME Journal. 2023 Oct 14. (IF: 11.0)

17. Shen X, Shen D, Shao X, Niu B, He Y, Qian G*. (2023) Bactericidal T4SS-guided TA finder: a simple and powerful approach to discover new type II toxin-antitoxin systems. mLife. 2023, 2(3), 295–307.

18. Lin L, Li L, Tao M, Wu Q, Zhou L, Wang B, Wang L, Shao X, Zhong C, Qian G*. (2023) Assembly of an active microbial consortium by engineering compatible combinations containing foreign and native biocontrol bacteria of kiwifruit. CSB Journal. Volume 21, 2023, Pages 3672-3679. (IF: 6.0)

19. Liao J, Li Z, Xiong D, Shen D, Wang L, Shao X, Li T, Qian G*. (2022) A novel and efficient platform for discovering non-canonical quorum-quenching proteins. Microbiology Spectrum. (IF: 9.0)

20. Xu K, Wang L, Xiong D, Chen H, Tong X, Shao X, Li T, Qian G*. (2022) The Wsp chemosensory system modulates c-di-GMP-dependent biofilm formation by integrating DSF quorum sensing through the WspR-RpfG complex in Lysobacter. NPJ Biofilms Microbiome. (IF: 8.5)

21. Wu, Q., Wang, B., Shen, X., Shao X, Qian G*. (2021) Unlocking the bacterial contact-dependent antibacterial activity to engineer a biocontrol alliance of two species from natural incompatibility to artificial compatibility. Stress Biology, 1,19.

22. Wang B#, Li L#, Lin Y#, Shen D, Shao X, Zhong C*, Qian G*. (2022) Directed isolation of biocontrol agents from plants through plant-pathogen co-culturing and pathogen enrichment. Phytopathology Research, 4:19. (IF: 3.955)

23. Xie Y #, Li J#, Ding Y#, Shao X, Sun Y, Xie F, Liu S,Tang S, Deng X*. (2022) An atlas of bacterial two-component systems reveals function and plasticity in signal transduction. Cell Reports. 41(3):111502. (IF: 9.4)

24. Sun Y., Shao X, Zhang Y. Han L, Huang J, Liu J, Deng X*. (2022) Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation. Cell & Bioscience, 12, 147. (IF: 7.5)

25. Xie Y, Ding Y, Shao X, Yao C, Li J, Liu J, Deng X*. (2021) Pseudomonas syringae Senses Polyphenol via Phosphorelay Crosstalk to Inhibit Virulence. EMBO Reports. 2021 Dec 6;22(12):e52805.（IF: 8.8)

26. Cai Z, Yang F, Shao X, Yue Z, Li Z, Song Y, Pan X, Jin Y, Cheng Z, Ha UH, Feng J, Yang L, Deng X, Wu W, Bai F*. (2022) ECF Sigma Factor HxuI Is Critical for In Vivo Fitness of Pseudomonas aeruginosa during Infection. Microbiology Spectrum. 2022 Feb 23;10(1):e0162021. (IF: 9.0)

27. Xie Y, Shao X, Zhang Y, Liu J, Wang T, Zhang W, Hua C, Deng X*. (2019). Pseudomonas savastanoi Two-Component System RhpRS Switches between Virulence and Metabolism by Tuning Phosphorylation State and Sensing Nutritional Conditions. mBio. 10 (2), 02838-18. (IF: 7.9）

28. Xie Y, Shao X, Deng X*. (2019) Regulation of Type III Secretion System in Pseudomonas syringae. Environmental Microbiology. (21)12:4465-4477. (IF: 5.5)

29. Hua C, Wang T, Shao X, Xie Y, Hao H, Li, J, Zhang W, Zhang Y, Ding Y, Jiang L, Wang X, Deng, X*. (2020). Pseudomonas syringae dual-function protein Lon switches between virulence and metabolism by acting as both DNA-binding transcriptional regulator and protease in different environments. Environmental Microbiology. (22)7:2968-2988. (IF: 5.5)

30. Wang T#, Cai Z#, Shao X, Zhang W, Xie Y, Zhang Y, Hua C, Schuster S C, Yang L, Deng X*. (2019) The Pleiotropic Effects of c-di-GMP Content in Pseudomonas syringae. Applied Environmental Microbiology. 85 (10) e00152-19. (IF: 4.8)

31. Zhang Y #, Zhou C #, Pu Q, Wu Q, Tan S, Shao X, Zhang W, Xie Y, Li R, Yu X, Wang R, Zhang L, Wu M*, Deng X*. (2019) Pseudomonas aeruginosa regulatory protein AnvM controls pathogenicity in anaerobic environments and impacts host defense. mBio. 10 (4): e01362-19. pii: e01362-19. (IF: 7.9)

32. Fan L, Wang T, Hua C, Sun W, Li X, Grunwald L, Liu J, Wu W, Shao X, Yin Y, Yan J*, Deng X*. (2020) A Compendium of DNA-Binding Specificities of Transcription Factors in Pseudomonas syringae. Nature Communications. 2020 Oct 2;11(1):4947.  (IF: 12.1)

33. Zhou T, Yin C, Zhang Y, Shi H, Wang J, Sun L, Shao X, Gao R, Wang W*, Deng X*. (2016) Lon protease is involved in RhpRS-mediated regulation of type III secretion in Pseudomonas syringae. Mol Plant Microbe Interact. 29(10): 807-814. (IF: 4.2）

34. Gao R, Shi H, Zhang Y, Shao X, Deng X. (2016) N6-methyladenosine Modification in Bacterial mRNA. Clin Microbiol 5: 256.

35. Wang B., Shao X., Qian G. (2023). Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope. In: Minamino, T., Miyata, M., Namba, K. (eds) Bacterial and Archaeal Motility. Methods in Molecular Biology, vol 2646. Humana, New York, NY.