个人简介
黄彦,女,江苏盐城人,副教授,硕士生导师。目前主要开展微生物新型酶资源的挖掘及应用研究,酶分子的晶体结构解析及催化机理研究。主持在研国家自然科学基金面上项目,国家重点研发计划项目子课题,以第一作者或通讯作者在Appl Environ Microbiol,FOODs,Int J Biol Macromol,Int J Mol Sci等期刊发表论文近20篇。
联系方式
电子邮箱:huangyan@njau.edu.cn
办公地址:生命科学楼C5016
工作经历
2015/01- 南京农业大学,微生物系,副教授
2012/06-2014/12, 南京农业大学,微生物系,讲师
2012/12-2017/06, 南京农业大学,生物学,博士后
教育经历
2007/09–2012/06,中国药科大学,微生物与生化药学,硕博
2003/09–2007/06,南京中医药大学,生物制药,本科
教学情况
承担本科生“生命科学概论”、“普通生物学”、“基础微生物学实验”、“微生物学实验”和“微生物生理学实验”等课程教学。
科研情况
发表论文
1. Liu H, Huang M, Wei S, Wang X, Zhao Y, Han Z, Ye X, Li Z, Ji Y, Cui Z, Huang Y*. Characterization of a multi-domain exo-β-1,3-galactanase from Paenibacillus xylanexedens. Int J Biol Macromol. 2024, 266:131413.
2. Zhong L, Guo X, Xue H, Qiao Y, Mao D, Ye X, Cui Z, Li Z, Hu G, Huang Y*. Quality Characteristics of Reduced-Fat Emulsified Sausages Made with Yeast Mannoprotein Enzymatically Prepared with a β-1,6-glucanase. Foods, 2023, 26;12(13):2486.
3. Chen SB, Zhang H, Chen S, Ye XF, Li ZK, Liu WD, Cui ZL, Huang Y*. Structural and Functional Characterization of a New Bacterial Dipeptidyl Peptidase III Involved in Fruiting Body Formation in Myxobacteria. Int J Mol Sci, 2023, 24:631.
4. Wang X, Chen S, Fan L, Liu H, Chen Si, Ye X, Li Z, Cui Z, Huang Y*. Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18. Protein Expr Purif, 2022, 194:106074.
5. Zhong L#, Wang X#, Fan L, Ye X, Li Z, Cui Z, Huang Y*. Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing. Protein Expr Purif, 2021, 179:105798.
6. Zhang Y, Duan Y, Zhong L, Li Z, Cui Z, Huang Y*. Characterization of a novel aldo-keto reductase with anti-Prelog stereospecificity from Corallococcus sp. EGB. Int J Biol Macromol, 2020, 146:36-44.
7. Ye X, Li Z, Luo X, Wang W, Li Y, Li R, Zhang B, Qiao Y, Zhou J, Fan J, Wang H, Huang Y, Cao H, Cui Z*, Zhang R*. A predatory myxobacterium controls cucumber Fusarium wilt by regulating the soil microbial community. Microbiome, 2020, 8:49.
8. Li Z, Ye X, Liu M, Xia C, Zhang L, Luo X, Wang T, Chen Y, Zhao Y, Qiao Y, Huang Y, Cao H, Gu X, Fan J, Cui Z*, Zhang Z*. A novel outer membrane β-1,6-glucanase is deployed in the predation of fungi by myxobacteria. ISME J, 2019, 13(9):2223-2235.
9. Zhou J, Li Z, Zhang H, Wu J, Ye X, Dong W, Jiang M, Huang Y*, Cui Z*; Novel maltogenic amylase CoMA from Corallococcus sp strain EGB catalyzes the conversion of maltooligosaccharides and soluble starch to maltose, Appl Environ Microbiol, 2018, 84(14):UNSP e00152-18.
10. Huang Y, Zhang H, Ben P, Duan Y, Lu M, Li Z, Cui Z*; Characterization of a novel GH36 alpha-galactosidase from Bacillus megaterium and its application in degradation of raffinose family oligosaccharides, Int J Biol Macromol, 2018, 1: 98-104.
11. Huang Y, Duan Y, Zhang Y, Fan P, Li Z, Liu W, Cui Z*. Crystal structure of hydroxyquinol 1,2-dioxygenase PnpC from Pseudomonas putida DLL-E4 and its role of N-terminal domain for catalysis, Biochem Biophys Res Commun, 2018, 507(1-4): 267-273.
12. Huang Y, Tu H, Zheng W, Duan Y, Li Z, Cui Z*. PnpB involvement in the regulation of temperature-sensitive para-nitrophenol degradation in Pseudomonas putida MT54 via PnpA. Biochem Biophys Res Commun. 2018 503(3):1575-1580.
13. Sun Z, Qin R, Li D, Ji K, Wang T, Cui Z, Huang Y*. A novel bacterial type II l-asparaginase and evaluation of its enzymatic acrylamide reduction in French fries. Int J Biol Macromol, 2016, 92:232-239.
14. Xin Y, Sun Z, Chen Q, Wang J, Wang Y, Luogong L, Li S, Dong W, Cui Z, Huang Y*. Purification and characterization of a novel extracellular thermostable alkaline protease from Streptomyces sp. M30. J Microbiol Biotechnol, 2015, 25(11):1944–1953.
15. Li JQ#, Huang Y#, Hou Y, Li X, Cao H, Cui ZL*. Novel gene clusters and metabolic pathway involved in 3,5,6-trichloro-2-pyridinol degradation by Ralstonia sp. strain T6, Appl Environ Microbiol, 2013, 79(23), 7445-7453.
16. Lu ML#, Huang Y#, White MA#, Wu XR, Liu N, Cheng XD*, Chen YJ*. Dual catalysis mode for the dicarbonyl reduction catalyzed by diketoreductase, Chem Commun, 48(92), 2012, 11352-11354.
17. Huang Y, Lu Z, Liu N, Chen YJ*. Identification of important residues in diketoreductase from Acinetobacter baylyi by molecular modeling and site-directed mutagenesis, Biochimie, 2012, 94(2), 471-478.
18. Huang Y, Lu Z, Ma M, Liu N, Chen YJ*. Functional roles of Tryptophan residues in diketoreductase from Acinetobacter baylyi, BMB Rep, 2012, 45(8), 452-457.
19. Huang Y, Liu N, Wu XR, Chen YJ*. Dehydrogenases/Reductases for the synthesis of chiral pharmaceutical intermediates, Curr Org Chem, 2010, 14(14), 1447-1460.
科研项目
1. 国家自然科学基金面上项目,32270066,麦芽六糖淀粉酶AmyM的产物专一性及生淀粉结合机制研究,2023/01-2026/12,主持。
2. 国家重点研发计划项目,2020YFC1806800,场地污染微界面行为原位表征技术与方法(课题2 土壤微界面复合污染过程多技术方法研究),2020/09-2024/08,子课题主持。
3. 国家重点研发计划项目,2019YFA09005200,人造蛋白质合成的细胞设计构建及应用(课题3 细胞工厂全网络调控与功能蛋白的高效合成),2020/01-2024/12,子课题主持。
4. 亚热带农业生物资源保护与利用国家重点实验室开放课题,SKLCUSA-b201818,粘细菌来源的激发子鉴定及其诱导植物免疫反应的机制研究,2019/01-2020/12,主持。
5. 农业农村部农业微生物资源收集与保藏重点实验室开放课题,MRCP-2018-4,捕食性粘细菌资源收集及其对水稻真菌病害防治作用的研究,2019/01-2020/12,主持。
6. 国家自然科学基金青年项目,31400056,粘细菌新型β-1,3-葡聚糖酶的催化机制研究,2015/01-2017/12,主持。
7. 江苏省自然科学基金青年项目,BK20140687,粘细菌多功能 β-1,3-葡聚糖酶的结构与催化机理研究,2014/07-2017/06,主持。
8. 中国博士后科学基金面上项目,2013M541685,硝磺草酮高抗性HPPD基因的筛选、克隆及抗性机制研究,2013/10-2014/12,主持。
9. 江苏省博士后科研资助项目,1302143C,除草剂环磺酮高抗性HPPD基因的资源发掘及其抗性机制研究,2013/12-2014/12,主持。
10. 中央高校基本业务费青年项目,KJQN201528,粘细菌新型β-1,3-葡聚糖酶的催化机制研究,2015/01-2017/12,主持。
申请专利
1. 黄彦,耿志涛,蔡丽娜,黄敏,多拷贝串联与高密度发酵合成贻贝蛋白的方法,申请号:202410786349.6
2. 黄彦,陈思博,崔中利,李周坤,叶现丰,一种检测对硝基苯酚的微生物荧光传感器及其制备方法和应用,申请号:202210937245.1
3. 崔中利,董维亮,黄彦,侯颖,王飞,苯并噁唑酮类化合物水解酶、编码该酶的基因、含有该基因的工程菌及其应用,申请号:201510398472.1
4. 崔中利,李周坤,黄彦,一种珊瑚球菌及其应用. 申请号:201310028459.8
5. 崔中利,李周坤,黄彦,一种α-淀粉酶及其基因、含有该基因的工程菌及其应用,申请号:ZL 201310043628.5
科研奖励
1. 双羰基还原酶的发现、催化机制及药物生物合成应用,2019年度江苏省科学技术二等奖,陈依军,吴旭日,陆美玲,黄彦,刘楠,王淑珍。
南京农业大学生命科学学院 教学科研
南京农业大学生命科学学院 教学科研
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