李家松

Professor ,Doctoral Supervisor ,Master Tutor

李家松，理学博士，南京农业大学生命科学学院，教授，博士生导师

电子邮箱：jiasong.li@njau.edu.cn；地址：生命科学楼B5014

ORCID：https://orcid.org/0000-0003-0341-2038

2014年在中国科学技术大学获博士学位，2015-2024年先后在耶鲁大学、德克萨斯大学圣安尼奥分校担任博士后和研究助理教授，2024年入选“江苏省特聘教授”并加入南京农业大学生命科学学院担任课题组组长。在美期间，长期从事化学与生物的交叉学科研究，在金属酶的结构与功能领域做出了具有重要影响的原创性贡献。研究工作主要发表在Nat. Chem. Biol.、J. Am. Chem. Soc.、Angew. Chem. Int. Engl.、Biochemistry等国际高水平学术期刊上。近期工作如下：

钴(III)-超氧化物复合物揭示硫醇双加氧酶催化机制（https://www.x-mol.com/news/894603）

吲哚-N-过氧化修饰对结核分枝菌金属酶KatG功能的调控（https://www.x-mol.com/news/901765）

基因编码的二氟代酪氨酸在酶催化机理方面的应用（https://www.x-mol.com/news/507046）

课题组研究方向简介：

未来研究方向将聚焦于环境微生物相关金属酶的催化机制研究。本课题组主要结合蛋白质晶体学（Protein crystallography）、电子顺磁共振（EPR）、共振拉曼光谱（rR）、液相色谱-质谱联用（LC-MS）、核磁共振（NMR）、酶定向进化（Directed evolution）以及基因密码子拓展技术（Genetic code expansion），开展以下两方面的研究：

探究环境微生物相关新型金属酶的催化机制；
通过酶定向进化对金属酶进行改造，以优化其在有机污染物降解中的应用。

在微生物学系/生化与分子系招收研究生，欢迎报考本课题组。

代表作

1. Li J., Duan R., Traore, E., Nguyen R. C., Davis I., Griffth W. P., Goodwin D. C., Jarzecki A. A., Liu A. Indole-N-linked hydroperoxyl adduct of protein-derived cofactor modulating catalase-peroxidase functions. Angew. Chem. Int. Ed. 2024, e202407018. (DOI: 10.1002/anie.202407018)

2. Li J*., Duan R., Liu A*. Cobalt(II)-substituted cysteamine dioxygenase proceeds through a cobalt(III)-superoxo complex. J. Am. Chem. Soc., 2024, 146, 27, 18292–18297. (DOI: 10.1021/jacs.4c01871)

3. Li J., Davis I., Liu A. Formation of monofluorinated radical cofactor in galactose oxidase through copper-mediated C−F bond scission. J. Am. Chem. Soc., 2020, 142, 44, 18753–18757. (DOI: 10.1021/jacs.0c08992)

4. Li J., Koto T., Davis I., Liu A. Probing the Cys-Tyr cofactor biogenesis in cysteine dioxygenase by the genetic incorporation of fluorotyrosine. Biochemistry. 2019, 58, 17, 2218-2227. (DOI: 10.1021/acs.biochem.9b00006)

5. Li J., Griffith WP, Davis I., Shin I., Wang J., Li F., Wang Y., Wherritt D., and Liu A* Cleavage of a carbon–fluorine bond by an engineered cysteine dioxygenase. Nature chemical biology, 2018, 14, 9, 853-860. (DOI: 10.1038/s41589-018-0085-5)

其他论文

6. Liu, X.,[+] Jiang, L.,[+] Li, J.,[+] Wang, L., Yu, Y., Zhou, Q., ... & Wang, J. Significant expansion of fluorescent protein sensing ability through the genetic incorporation of superior photo-induced electron-transfer quenchers. J. Am. Chem. Soc., 2014, 136(38), 13094-13097. (Co-first author, DOI: 10.1021/ja505219r)

7. Liu, X.,[+] Li, J.,[+] Hu, C., Zhou, Q., Zhang, W., Hu, M., Zhou, J. and Wang, J. Significant expansion of the fluorescent protein chromophore through the genetic incorporation of a metal-chelating unnatural amino acid. Angew. Chem. Int. Ed. 2013, 52: 4805–4809. (Co-first author, DOI: org/10.1002/anie.201301307)

8. Li, F,[+] Shi, P.,[+] Li, J.,[+] Yang, F., Wang, T., Zhang, W., Gao, F., Ding, W ., Li, D., Li, J., Xiong, Y., Sun, J., Gong, W ., Tian, C. and Wang, J. A genetically encoded 19F NMR probe for tyrosine phosphorylation. Angew. Chem. Int. Ed., 2013, 52: 3958–3962. (Co-first author, DOI: 10.1002/anie.201300463)

9. Liu, X.,[+] Li, J.,[+] Dong, J.,[+] Hu, C., Gong., and Wang, J. Genetic incorporation of a metal- chelating amino acid as a probe for protein electron transfer. Angew. Chem. Int. Ed., 2012, 51:10261–10265. (Co-first author, DOI: 10.1002/anie.201204962)

会议报告

Oct. 2024 Speaker, Southwest Regional Meeting of the ACS (SWRM), Waco, TX.

Feb. 2023 Speaker, Research Core Facility Fair, UTSA, San Antonio, TX.

Jul. 2017 Speaker, GRC— Enzymes, Coenzymes & Metabolic Pathways, NH.

Oct. 2013 Speaker, the 4th National "Interdisciplinary Protein Research" Symposium., CN.